mouse anti ll37 antibody  (Santa Cruz Biotechnology)

Journal: Journal of Translational Medicine

Article Title: Conformational changes in myeloperoxidase induced by ubiquitin and NETs containing free ISG15 from systemic lupus erythematosus patients promote a pro-inflammatory cytokine response in CD4 + T cells

doi: 10.1186/s12967-020-02604-5

Figure Lengend Snippet: NETs from SLE patients are enriched in ISG15 and H2B is the main substrate. ISG15 and H2B expression was assessed in spontaneous and LPS induced NETs from SLE patients (n = 6) and healthy controls (n = 3) by indirect immunofluorescence (40X) and confocal microscopy. Spontaneous NETs from a representative SLE patient shows the presence of extracellular ISG15 in the NET ( a ). In contrast to the SLE sample, a representative healthy control image shows the absence of ISG15 in the NET ( b ). Blue (DNA), red (ISG15) and green (H2B). Cumulative data from SLE patients and healthy controls (n = 6 subjects per group) show increased expression of ISG15 in the SLE samples ( c ). In SLE samples, ISG15 and H2B colocalized inside NETs with a high Pearson (R = 0.81) and Costes p value (1.0) in a representative SLE patient ( d ) and boxes represent pooled data (n = 6 subjects per group) that show increased colocalization of SLE samples vs healthy controls ( e ). H2B with ISG15 had the higher colocalization R value compared to other NET proteins such as LL37 or HMGB1 (Additional file : Figures S5 and S6). * p < 0.05

Article Snippet: Additionally, we used mouse anti-human LL37 (LSBio™) or mouse anti-human HMGB1 ( Thermo Fisher™ ). (Additional file : Figures S5 and S6).

Techniques: Expressing, Immunofluorescence, Confocal Microscopy